Gene Expression Regulation by Upstream Open Reading Frames and Human Disease

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Gene Expression Regulation by Upstream Open Reading Frames and Human Disease

Upstream open reading frames (uORFs) are major gene expression regulatory elements. In many eukaryotic mRNAs, one or more uORFs precede the initiation codon of the main coding region. Indeed, several studies have revealed that almost half of human transcripts present uORFs. Very interesting examples have shown that these uORFs can impact gene expression of the downstream main ORF by triggering ...

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We engineered short ORFs and used them to control the expression level of recombinant proteins. These short ORFs, encoding a two-amino acid peptide, were placed upstream of an ORF encoding a protein of interest. Insertion of these upstream ORFs (uORFs) resulted in suppression of protein expression. By varying the base sequence preceding the uORF, we sought to vary the translation initiation rat...

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Department of Environmental and Biomolecular Systems, Oregon Health and Science University, Beaverton, Oregon 97006, USA; Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97201, USA; Divisions of Human Biology and Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA; Department of Microbiology and De...

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ژورنال

عنوان ژورنال: PLoS Genetics

سال: 2013

ISSN: 1553-7404

DOI: 10.1371/journal.pgen.1003529